English translation for "clone method"
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- 克隆方式；复制方法
Related Translations:
cloning: 基因转殖 克隆克隆繁殖克隆技术克卢克隆化无性繁殖系化无性系化
- Example Sentences:
| 1. | To acquire the polyclonal antibody of hbaf53 , we made use of several molecular cloning methods
为了获得hbaf53的多克隆抗体，本实验采用基因工程的方法制备抗原。 | | 2. | The traditional homogene cloning method was modified on two aspects : first , homo - primers was designed at the sites with minimum degeneracy instead of maximum homology , secondly , some " n " bases were replaced with inosine in degenerate codes , resulting hi the degeneracy reduced from over 1000 times to below 100 times
改进后的同源引物简并度从1000倍以上降低至100倍以下，利用rt一pcr成功地扩增出一条630bp的盐藻cdna片段，该片段与其它物种的烯醇酶基因具有很高的相似性。 | | 3. | The complete nucleotide sequences of two strain viruses were determined . ten pairs of primers were designed based on the nucleotide sequences of tbev sofjin - ho , oshima5 - 10 by dnastar software . the virus cdna was expanded by rt - pcr in ten parts . sequenced by clones method . 5 " terminal and 3 " terminal cdna was expanded by race method . the nucleotide sequences and deduced amino acid sequences of two strain viruses were compared with the sequences in genbank by dnastar software
根据genbank中已公布的tbevsofjin - ho株和oshima5 - 10株核苷酸序列利用dnastar软件设计10对引物，利用rt - pcr技术分段扩增病毒cdna ，克隆法测序。病毒基因组5末端和3末端序列采用race法扩增。测序结果用dnastar软件拼接，在genbank中检索6株tbev编码区核苷酸序列、 12株tbeve蛋白核苷酸序列，并在dnastar上进行核苷酸序列、由此推导的氨基酸序列比较及构建系统发生树。 | | 4. | Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
将培养10h的化学去核卵母细胞与供体成纤维细胞融合后lh 、 2h 、 3h ，分别有77 . 6 % 、 70 . 6 % 、 58 . 9 %重构胚的染色质发生凝集，其余胚胎的染色体则处于原核期;而只在融合后3h ， 27 . 9 %重构胚被标记出组装的纺锤体，且其中的同源染色体己经分离。 |
- Similar Words：
- "clone crayon" English translation, "clone culture" English translation, "clone deletion theory" English translation, "clone marker" English translation, "clone market" English translation, "clone mode" English translation, "clone molecular dna" English translation, "clone money market fund" English translation, "clone oil paint" English translation, "clone paintbrush" English translation
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